|
KMID : 0363620090300030001
|
|
Journal of Korean Oriental Medicine
2009 Volume.30 No. 3 p.1 ~ p.14
|
|
|
Effects of Woo-Gui-Um on Aβ Toxicity and Memory Dysfunction in Mice
|
|
Hwang Gwang-Ho
Kim Bum-Hoi
Shin Jung-Won
Shim Eun-Sheb
Lee Dong-Eun
Lee Sang-Yul
Lee Hyun-Sam
Jung Hyuk-Sang
Sohn Nak-Won
Sohn Young-Joo
|
|
|
Abstract
|
|
|
Objectives : Alzheimer’s disease (AD) is characterized by neuronal loss and extracellular senile plaque. Moreover, the cellular actions of β-amyloid (Aβ) play a causative role in the pathogenesis of AD. This study was designed to determine whether Woo-Gui-Um, a commonly used Korean herbal medicine, has the ability to protect cortical and
hippocampal neurons against Aβ25-35 neurotoxicity
Methods : In the present study, the authors investigated the preventative effects of the water extract of Woo-Gui-Um in a mouse model of AD. Memory impairment was induced by intraventricularly (i.c.v.) injecting Aβ25-35 peptides
into mice. Woo-Gui-Um extract was then administered orally (p.o.) for 14 days. In addition, Aβ25-35 toxicity on the
hippocampus was assessed immunohistochemically, by staining for Tau, MAP2, TUNEL, and Bax, and by performing
an in vitro study in PC12 cells.
Results : Woo-Gui-Um extract had an effect to improve learning ability and memory score in the water maze task.
Woo-Gui-Um extract had significant neuroprotective effects in vivo against oxidative damage and apoptotic cell death
of hippocampal neurons caused by i.c.v. Aβ25-35. In addition, Woo-Gui-Um extract was found to have a protective
effect on Aβ25-35-induced apoptosis, and to promote neurite outgrowth of nerve growth factor (NGF)-differentiated
PC12 cells.
Conclusions : These results suggest that Woo-Gui-Um extract reduces memory impairment and Alzheimer’s dementia via an anti-apoptotic effect and by regulating Tau and MAP2 in the hippocampus.
|
|
|
KEYWORD
|
|
|
Woo-Gui-Um, learning and memory, Aβ25-35, Tau, MAP2, Bax
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|